Osmosis Data Sheet

Data Sheet: Activity - Osmosis

Name	Course	Date

Activity Data Code      

Procedure I -Test Solution 1: Water

Complete the tablesand questions below using your data and information found under the Background tab

Data Table INote: Difference in Final Volumes = Final Volume of Test Sol - Final Volume of Water

Trial	Starting Volume of Test Solution (L)	Starting Volume of Water (L)	Final Volume of Test Solution (L)	Final Volume of Water (L)	Difference in Final Volumes (L)
1	1.28	1.75	1.51	1.52	-0.01
2	1.28	2.00	1.64	1.64	0

Observations and Questions

[1] Given that the final heights (and volumes) are the same for the water and test solution, what can you conclude about the amount of solutes in these two solutions? Explain your reasoning.

[2] As discussed in the Background material, water is an important biological molecule. Do you expect water to continue to flow across the semipermeable; lipid bilayer after osmotic equilibrium is reached? Why or why not?

Procedure II -Test Solution 2: Guanine solution

Complete the tables and questions below using your data and information found under the Background tab

Data Table IINote: Difference in Final Volumes = Final Volume of Test Sol - Final Volume of Water

Trial	Starting Volume of Test Solution (L)	Starting Volume of Water (L)	Final Volume of Test Solution (L)	Final Volume of Water (L)	Difference in Final Volumes (L)
1	1.28	1.50	1.82	0.96	0.86
2	1.28	2.00	2.07	1.21	0.86

Observations and Questions

[3] Why did the height (and volume) change in the test solution? What is the basis for the increase in the volume in the test solution?

[4] What do you think would happen if both starting volumes were the same? Test your hypothesis by doing a data run. Explain your observation.

[5] Why is the difference in final volumes the same for both trials?

Procedure III -Test Solution 3: Cytochrome C solution

Complete the table and questions below using your data and information found under the Background tab (see the Summary of Needed Formulas section) and Activity Form tab

Data TableIIINote: Difference in Final Volumes = Final Volume of Test Sol - Final Volume of Water

Trial	Starting Volume of Test Solution (L)	Starting Volume of Water (L)	Final Volume of Test Solution (L)	Final Volume of Water (L)	Difference in Final Volumes (L)
1	1.28	2.00	1.97	1.31	0.66
2	1.28	2.25	2.10	1.43	0.67

Observations and Questions

[6] Based on your data and observations from procedure II and procedure III, which solution has the highest concentration? Explain your answer.

[7] Concentration Calculation: Using your Trial 1 data from procedure II and procedure III, and the known concentration of guanine (see Activity Form tab), calculate the concentration of the cytochrome c solution. Hint: See the Summary of Needed Formulas section under the Background tab and information found under the Activity Form tab.